Culturing Fruit Flies

fruit fly


1. Mix ¾ cup of boiling RO Water/bottled water with ½ cup of Josh’s Frogs Fruit Fly media, or follow packaging directions for Repashy SuperFly.

2. Add a handful of excelsior to the cup.

3. Cover the cup and wait for it to cool.

4. Add 50-100 flies. Cultures should be kept below 85 degrees and will start producing flies in 12-14 days. If the cultures seem to be drying out (or molding), spray with RO Water and use more water when making cultures.

Josh’s Frogs recommends keeping the cultures in a plastic drawer system to prevent them from drying out. This media contains a mold inhibitor. Shake well before using.